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. 2019 Sep 20;12:224. doi: 10.3389/fnmol.2019.00224

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Copyright © 2019 Sharaf, Mensching, Keller, Rading, Scheffold, Palkowitsch, Djogo, Rezgaoui, Kestler, Moepps, Failla and Karsak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–G) Confocal light microscopy of HEK293 cells transiently expressing CB2 receptors. (A) Immunohistochemical staining of CB2 (green), p62 (red), and DAPI (blue). The membrane was stained by Texas-red conjugated wheat-germ-agglutinin (white) and DAPI revealed the localization of the nucleus. (B–G) 3D reconstruction modeling of confocal images using a segmentation algorithm to distinguish single signal from different channels. (D–G) Higher zoom in of 3D reconstructed cell showing a strong overlap of CB2 positive areas with the membrane marker (white). P62 was found in vesicular like structures that intermingled with membranal CB2 positive areas (yellow arrows).