FIGURE 5.

(A) Schematic drawing of CB2 receptor protein with extracellular N-terminus and three extracellular loops (e1, e2, e3), seven transmembrane domains, and three intracellularly localized loops (i1, i2, i3) and the C-terminus. The red marked intracellular regions were deleted in CB2 expression plasmids and used for following Co-IP studies. (B) Representative Western blot results of CB2 precipitations using Flag antibody with lysates of HEK293 cells transiently transfected with p62-WT (1 μg) and Flag-CB2 constructs with the respective deletions (ΔC-CB2, Δ i1-CB2, Δ i2-CB2, Δ i3-CB2). Considering lower expression levels of deletion constructs compared to WT-full length Flag-CB2, the co-precipitated p62 protein levels were similar in all approaches. The IP figure was grouped from cropped parts of the same gels/blots indicated by the border line. This experiment has been performed at least two times independently.