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. 2019 Sep 20;12:224. doi: 10.3389/fnmol.2019.00224

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Copyright © 2019 Sharaf, Mensching, Keller, Rading, Scheffold, Palkowitsch, Djogo, Rezgaoui, Kestler, Moepps, Failla and Karsak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic illustration of Flag-p62 deletion constructs for ZZ, PEST, and UBA motifs that were used for following co-immunoprecipitation experiments to identify interaction region. (B) Stably expressing HA-CB2 HEK293 cells were transiently transfected with different Flag-p62-deletion constructs, respectively. Protein extracts were used for precipitation of Flag-p62 using Flag antibody. The expression levels in input samples for CB2 and p62 were comparable in all constructs. Immunoprecipitation result of Flag-p62 ΔZZ revealed a significantly reduced coprecipitation of CB2 indicating that this domain in p62 is involved in the interaction with CB2. Representative results of three independent experiments were presented.