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. 2019 Sep 20;12:224. doi: 10.3389/fnmol.2019.00224

TABLE 1.

Sequences of primer pairs used for the generation of CB2 deletion constructs of intracellular loops (Δi) and p62 deletion constructs.

Flag-plasmids Forward primer (5′–3′) Reverse primer (5′–3′)
Δi1-CB2 GTGCTCTATCTGATCCTGCTGTTCATTGGCAGCTTG CAAGCTGCCAATGAACAGCAGGATCAGATAGAGCAC
Δi2-CB2 CCTCCTGCTGACCGCCATTGCACTGGTGA TCACCAGTGCAATGGCGGTCAGCAGGAGG
Δi3-CB2 GGGCATGTTCTCTGGACCCTAGGGCTAGTG CACTAGCCCTAGGGTCCAGAGAACATGCCC
p62-ΔZZ122-167 AGGGGCTGGGGAACATGTTGCGGGGC GCCCCGCAACATGTTCCCCAGCCCCT
P62-ΔPEST266-294 CCCACCCGGCTTTCTTTTCCCTCCGTGCT AGCACGGAGGGAAAAGAAAGCCGGGTGGG
P62-ΔUBA389-434 CACATCTCCCGCCAAAGCATCCCCCGCC GGCGGGGGATGCTTTGGCGGGAGATGTG