TABLE 2.
CB2 interactors in HEK293 cells.
| Entry | Entry name | Protein names | Unique | Sequence | Molecular | Ratio |
| peptides | coverage [%] | weight [kDa] | CB2/ctrl | |||
| P34972 | CNR2_HUMAN | Cannabinoid receptor 2 | 16 | 39.2 | 39.68 | 845.0 |
| Q15392 | DHC24_HUMAN | Delta(24)-sterol reductase | 2 | 5.4 | 60.101 | Only in CB2 |
| Q9Y394 | DHRS7_HUMAN | Dehydrogenase/reductase SDR family member 7 | 2 | 14.2 | 38.298 | Only in CB2 |
| Q9UJ14 | GGT7_HUMAN | Glutathione hydrolase 7 | 4 | 11.2 | 70.466 | Only in CB2 |
| Q5T447 | HECD3_HUMAN | E3 ubiquitin-protein ligase HECTD3 | 2 | 3.8 | 97.112 | Only in CB2 |
| Q8IYS2 | K2013_HUMAN | Uncharacterized protein KIAA2013 | 4 | 11.1 | 72.68 | Only in CB2 |
| O15162 | PLS1_HUMAN | Phospholipid scramblase 1 | 4 | 14.8 | 35.049 | Only in CB2 |
| O14967 | CLGN_HUMAN | Calmegin | 19 | 45.7 | 70.038 | 12388.6 |
| Q06136 | KDSR_HUMAN | 3-Ketodihydrosphingosine reductase (KDS reductase) | 7 | 32.2 | 36.187 | 2214.8 |
| Q96JJ7 | TMX3_HUMAN | Protein disulfide-isomerase TMX3 | 13 | 39 | 51.871 | 551.6 |
| Q99720 | SGMR1_HUMAN | Sigma non-opioid intracellular receptor 1 | 6 | 40.4 | 25.127 | 370.9 |
| P16615 | AT2A2_HUMAN | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) | 44 | 48.6 | 114.76 | 306.2 |
| P27824 | CALX_HUMAN | Calnexin (IP90) (Major histocompatibility complex class I antigen-binding protein p88) | 38 | 57.9 | 67.567 | 200.4 |
| Q8IWV7 | UBR1_HUMAN | E3 ubiquitin-protein ligase UBR1 | 3 | 2.8 | 200.21 | 170.0 |
| P37268 | FDFT_HUMAN | Squalene synthase | 8 | 24.5 | 48.115 | 168.0 |
| Q99442 | SEC62_HUMAN | Translocation protein SEC62 | 4 | 10 | 45.861 | 164.7 |
| O43505 | B4GA1_HUMAN | Beta-1.4-glucuronyltransferase 1 | 2 | 12.8 | 47.119 | 161.7 |
| Q9UBV2 | SE1L1_HUMAN | Protein sel-1 homolog 1 | 2 | 6.2 | 88.754 | 124.8 |
| P10586 | PTPRF_HUMAN | Receptor-type tyrosine-protein phosphatase F | 5 | 4.5 | 212.88 | 88.1 |
| O95260 | ATE1_HUMAN | Arginyl-tRNA–protein transferase 1 | 4 | 10.2 | 59.09 | 87.4 |
| Q96A33 | CCD47_HUMAN | Coiled-coil domain-containing protein 47 | 11 | 34.4 | 55.873 | 85.3 |
| O15269 | SPTC1_HUMAN | Serine palmitoyltransferase 1 | 4 | 11.8 | 52.743 | 77.3 |
| Q4ZIN3 | MBRL_HUMAN | Membralin (Transmembrane protein 259) | 3 | 7.3 | 67.888 | 62.9 |
| O43149 | ZZEF1_HUMAN | Zinc finger ZZ-type and EF-hand domain-containing protein 1 | 5 | 2.8 | 331.07 | 55.5 |
| Q13501 | SQSTM_HUMAN | Sequestosome-1 | 6 | 23.4 | 47.687 | 52.7 |
| O15258 | RER1_HUMAN | Protein RER1 | 2 | 15.3 | 22.958 | 51.5 |
| Q9H3N1 | TMX1_HUMAN | Thioredoxin-related transmembrane protein 1 | 3 | 12.9 | 31.791 | 42.2 |
| O00264 | PGRC1_HUMAN | Membrane-associated progesterone receptor component 1 | 8 | 57.4 | 21.671 | 40.5 |
| Q15043 | S39AE_HUMAN | Zinc transporter ZIP14 (LIV-1 subfamily of ZIP zinc transporter 4) | 2 | 6.7 | 54.212 | 31.2 |
| Q8NI60 | COQ8A_HUMAN | Atypical kinase COQ8A mitochondrial | 3 | 8.3 | 71.949 | 28.5 |
| P55084 | ECHB_HUMAN | Trifunctional enzyme subunit beta mitochondrial | 15 | 43.7 | 51.294 | 27.8 |
| P42356 | PI4KA_HUMAN | Phosphatidylinositol 4-kinase alpha | 2 | 1.6 | 231.32 | 26.3 |
| O75915 | PRAF3_HUMAN | PRA1 family protein 3 (ADP-ribosylation factor-like protein 6-interacting protein 5) | 2 | 16 | 21.614 | 25.6 |
| A1L0T0 | ILVBL_HUMAN | Acetolactate synthase-like protein | 4 | 10.9 | 67.867 | 23.1 |
| Q9Y4P3 | TBL2_HUMAN | Transducin beta-like protein 2 | 2 | 5.6 | 49.797 | 22.5 |
| O60884 | DNJA2_HUMAN | DnaJ homolog subfamily A member 2 | 2 | 6.8 | 45.745 | 22.2 |
| Q29963 | 1C06_HUMAN | HLA class I histocompatibility antigen. Cw-6 alpha chain (MHC class I antigen Cw∗6) | 5 | 21.9 | 40.968 | 21.5 |
| P20020 | AT2B1_HUMAN | Plasma membrane calcium-transporting ATPase 1 | 3 | 4.2 | 138.75 | 21.1 |
| P49755 | TMEDA_HUMAN | Transmembrane emp24 domain-containing protein 10 | 2 | 12.8 | 24.976 | 19.8 |
| P62341 | SELT_HUMAN | Thioredoxin reductase-like selenoprotein T (SelT) | 2 | 17.4 | 22.174 | 18.8 |
| P50395 | GDIB_HUMAN | Rab GDP dissociation inhibitor beta | 2 | 8.5 | 50.663 | 17.0 |
| P49411 | EFTU_HUMAN | Elongation factor Tu mitochondrial (EF-Tu) | 23 | 61.5 | 49.541 | 15.2 |
| Q8IXI1 | MIRO2_HUMAN | Mitochondrial Rho GTPase 2 (MIRO-2) (hMiro-2) | 2 | 4.4 | 68.117 | 15.2 |
| Q14257 | RCN2_HUMAN | Reticulocalbin-2 | 2 | 11 | 36.876 | 14.6 |
| Q9BQE3 | TBA1C_HUMAN | Tubulin alpha-1C chain (Alpha-tubulin 6) | 2 | 76.2 | 49.895 | 12.4 |
| Q9BXW9 | FACD2_HUMAN | Fanconi anemia group D2 protein (Protein FACD2) | 2 | 2.3 | 166.46 | 12.1 |
| P04844 | RPN2_HUMAN | Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit 2 | 11 | 37.9 | 69.283 | 12.1 |
| P04843 | RPN1_HUMAN | Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit 1 | 19 | 43 | 68.569 | 12.0 |
| P40939 | ECHA_HUMAN | Trifunctional enzyme subunit alpha mitochondrial | 27 | 53.1 | 82.999 | 12.0 |
| P39656 | OST48_HUMAN | Dolichyl-diphosphooligosaccharide–protein glycosyltransferase 48 kDa subunit | 11 | 45.2 | 50.8 | 11.9 |
| Q9BVA1 | TBB2B_HUMAN | Tubulin beta-2B chain | 3 | 67.2 | 49.953 | 11.5 |
| Q9BQB6 | VKOR1_HUMAN | Vitamin K epoxide reductase complex subunit 1 | 2 | 19 | 18.234 | 11.1 |
| P13797 | PLST_HUMAN | Plastin-3 (T-plastin) | 2 | 6.3 | 70.81 | 11.0 |
| Q9HCU5 | PREB_HUMAN | Prolactin regulatory element-binding protein | 3 | 15.1 | 45.468 | 10.8 |
| P07237 | PDIA1_HUMAN | Protein disulfide-isomerase (PDI) | 2 | 4.3 | 57.116 | 10.3 |
| P51648 | AL3A2_HUMAN | Fatty aldehyde dehydrogenase | 6 | 14.4 | 57.669 | 9.9 |
| P13073 | COX41_HUMAN | Cytochrome c oxidase subunit 4 isoform 1 mitochondrial | 2 | 13.6 | 19.576 | 9.0 |
| P08195 | 4F2_HUMAN | 4F2 cell-surface antigen heavy chain (4F2hc) | 3 | 7 | 71.122 | 9.0 |
| Q15363 | TMED2_HUMAN | Transmembrane emp24 domain-containing protein 2 (Membrane protein p24A) | 2 | 10.9 | 22.761 | 8.8 |
| P61204 | ARF3_HUMAN | ADP-ribosylation factor 3 | 2 | 18.2 | 20.601 | 8.8 |
| O15173 | PGRC2_HUMAN | Membrane-associated progesterone receptor component 2 | 2 | 18.8 | 23.818 | 7.9 |
| O95831 | AIFM1_HUMAN | Apoptosis-inducing factor 1 mitochondrial | 5 | 11.1 | 66.9 | 6.8 |
| P00403 | COX2_HUMAN | Cytochrome c oxidase subunit 2 | 3 | 16.3 | 25.565 | 6.1 |
| P05109 | S10A8_HUMAN | Protein S100-A8 (Calgranulin-A) | 3 | 32.3 | 10.834 | 6.0 |
| Q12931 | TRAP1_HUMAN | Heat shock protein 75 kDa mitochondrial (HSP 75) (TNFR-associated protein 1) | 7 | 13.2 | 80.109 | 5.5 |
| P22102 | PUR2_HUMAN | Trifunctional purine biosynthetic protein adenosine-3 | 4 | 7.5 | 107.77 | 5.4 |
| O14975 | S27A2_HUMAN | Very long-chain acyl-CoA synthetase (VLACS) (VLCS) | 2 | 5.5 | 70.311 | 5.2 |
| P11586 | C1TC_HUMAN | C-1-Tetrahydrofolate synthase cytoplasmic (C1-THF synthase) | 2 | 2.7 | 101.56 | 5.1 |
| O75396 | SC22B_HUMAN | Vesicle-trafficking protein SEC22b (ER-Golgi SNARE of 24 kDa) | 2 | 10.2 | 24.593 | 5.1 |
| P30101 | PDIA3_HUMAN | Protein disulfide-isomerase A3 | 5 | 13.9 | 56.782 | 5.1 |
| P53618 | COPB_HUMAN | Coatomer subunit beta (Beta-coat protein) | 2 | 2.2 | 107.14 | 4.9 |
| P51571 | SSRD_HUMAN | Translocon-associated protein subunit delta (TRAP-delta) | 2 | 18.5 | 18.998 | 4.8 |
| P61619 | S61A1_HUMAN | Protein transport protein Sec61 subunit alpha isoform 1 (Sec61 alpha-1) | 2 | 9 | 52.264 | 4.6 |
| P30048 | PRDX3_HUMAN | Thioredoxin-dependent peroxide reductase mitochondrial | 2 | 9.8 | 27.692 | 4.5 |
| Q7Z6Z7 | HUWE1_HUMAN | E3 ubiquitin-protein ligase HUWE1 | 2 | 0.5 | 481.89 | 4.3 |
| P50402 | EMD_HUMAN | Emerin | 3 | 17.3 | 28.994 | 4.3 |
| P24534 | EF1B_HUMAN | Elongation factor 1-beta (EF-1-beta) | 3 | 26.7 | 24.763 | 4.1 |
| Q53GQ0 | DHB12_HUMAN | Very-long-chain 3-oxoacyl-CoA reductase | 4 | 21.2 | 34.324 | 4.0 |
| Q9P035 | HACD3_HUMAN | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 | 9 | 33.1 | 43.159 | 3.8 |
| Q00325 | MPCP_HUMAN | Phosphate carrier protein mitochondrial (Phosphate transport protein) (PTP) | 8 | 28.5 | 39.958 | 3.7 |
| O14980 | XPO1_HUMAN | Exportin-1 | 5 | 8.3 | 123.38 | 3.7 |
| P06702 | S10A9_HUMAN | Protein S100-A9 (Calgranulin-B) | 3 | 36 | 13.242 | 3.7 |
| Q86VU5 | CMTD1_HUMAN | Catechol O-methyltransferase domain-containing protein 1 | 4 | 25.2 | 28.808 | 3.6 |
| Q93008 | USP9X_HUMAN | Probable ubiquitin carboxyl-terminal hydrolase FAF-X | 3 | 1.7 | 292.28 | 3.6 |
All specific interactors are classified by the identification in mass spectrometry data of triplicates by at least two unique peptides. The Uniprot accession numbers with protein name, descriptions and molecular weights are given. Sequence coverage by identified peptides is presented in %. Additionally, the ratio of identification in CB2 expressing cells to control cells was calculated.