TABLE 4.
Gene ontology (GO) functional annotation clustering.
| Count | p_Value | FDR | ||
| Cluster 1 | Enrichment score: 15.5 | |||
| UP_KEYWORDS | Endoplasmic reticulum | 34 | 2.7E-21 | 3.3E-18 |
| GOTERM_CC_DIRECT | Endoplasmic reticulum membrane | 27 | 3.2E-15 | 3.8E-12 |
| UP_SEQ_FEATURE | Topological domain:lumenal | 18 | 3.6E-12 | 4.9E-9 |
| Cluster 2 | Enrichment score: 9.61 | |||
| UP_KEYWORDS | Membrane | 63 | 1.3E-12 | 1.6E-9 |
| UP_KEYWORDS | Transmembrane helix | 54 | 4.0E-12 | 5.0E-9 |
| UP_KEYWORDS | Transmembrane | 54 | 4.6E-12 | 5.6E-9 |
| UP_SEQ_FEATURE | Transmembrane region | 48 | 1.1E-9 | 1.5E-6 |
| GOTERM_CC_DIRECT | Integral component of membrane | 50 | 2.7E-9 | 3.2E-6 |
| UP_SEQ_FEATURE | Topological domain:cytoplasmic | 33 | 3.1E-6 | 4.3E-3 |
| Cluster 3 | Enrichment score: 3.59 | |||
| UP_KEYWORDS | Oxidoreductase | 12 | 2.1E-5 | 2.6E-2 |
| GOTERM_BP_DIRECT | Oxidation–reduction process | 12 | 7.7E-5 | 1.1E-1 |
| GOTERM_MF_DIRECT | Oxidoreductase activity | 7 | 3.7E-4 | 4.7E-1 |
| UP_KEYWORDS | NADP | 5 | 6.8E-3 | 8.1E0 |
| Cluster 4 | Enrichment score: 3.17 | |||
| UP_KEYWORDS | Redox-active center | 7 | 4.4E-8 | 5.4E-5 |
| GOTERM_BP_DIRECT | Response to endoplasmic reticulum stress | 6 | 2.4E-5 | 3.4E-2 |
| GOTERM_BP_DIRECT | Cell redox homeostasis | 6 | 2.7E-5 | 3.9E-2 |
| INTERPRO | Thioredoxin domain | 5 | 3.0E-5 | 3.9E-2 |
| INTERPRO | Thioredoxin, conserved site | 4 | 5.2E-5 | 6.8E-2 |
| GOTERM_MF_DIRECT | Protein disulfide isomerase activity | 4 | 1.5E-4 | 1.9E-1 |
| INTERPRO | Thioredoxin-like fold | 6 | 2.6E-4 | 3.4E-1 |
| GOTERM_CC_DIRECT | Cell | 5 | 1.1E-3 | 1.3E0 |
| UP_SEQ_FEATURE | Short sequence motif:prevents secretion from ER | 4 | 2.1E-3 | 2.8E0 |
| GOTERM_MF_DIRECT | Isomerase activity | 3 | 4.4E-3 | 5.4E0 |
| UP_SEQ_FEATURE | Domain:thioredoxin | 3 | 8.2E-3 | 1.1E1 |
| UP_KEYWORDS | Isomerase | 3 | 8.7E-2 | 6.7E1 |
| UP_SEQ_FEATURE | Disulfide bond | 14 | 4.3E-1 | 1.0E2 |
| UP_KEYWORDS | Disulfide bond | 13 | 7.5E-1 | 1.0E2 |
| Cluster 5 | Enrichment score: 2.87 | |||
| GOTERM_MF_DIRECT | Long-chain-3-hydroxyacyl-CoA dehydrogenase activity | 3 | 6.6E-5 | 8.3E-2 |
| UP_KEYWORDS | Lipid metabolism | 9 | 3.6E-4 | 4.4E-1 |
| KEGG_PATHWAY | Fatty acid elongation | 4 | 5.4E-4 | 5.8E-1 |
| KEGG_PATHWAY | Fatty acid metabolism | 4 | 3.6E-3 | 3.8E0 |
| KEGG_PATHWAY | Biosynthesis of unsaturated fatty acids | 3 | 9.6E-3 | 9.9E0 |
| UP_KEYWORDS | Fatty acid metabolism | 4 | 1.4E-2 | 1.6E1 |
| Cluster 6 | Enrichment score: 2.56 | |||
| GOTERM_CC_DIRECT | Mitochondrial inner membrane | 9 | 8.2E-4 | 9.8E-1 |
| UP_SEQ_FEATURE | Transit peptide:mitochondrion | 9 | 8.5E-4 | 1.2E0 |
| UP_KEYWORDS | Transit peptide | 9 | 1.4E-3 | 1.7E0 |
| UP_KEYWORDS | Mitochondrion inner membrane | 6 | 4.7E-3 | 5.7E0 |
| GOTERM_CC_DIRECT | Mitochondrion | 14 | 6.3E-3 | 7.2E0 |
| UP_KEYWORDS | Mitochondrion | 11 | 1.4E-2 | 1.6E1 |
| Cluster 7 | Enrichment score: 2.54 | |||
| GOTERM_BP_DIRECT | Protein folding | 7 | 1.8E-4 | 2.5E-1 |
| UP_KEYWORDS | Chaperone | 5 | 8.9E-3 | 1.0E1 |
| GOTERM_MF_DIRECT | Unfolded protein binding | 4 | 1.5E-2 | 1.8E1 |
| Cluster 8 | Enrichment score: 2.38 | |||
| GOTERM_CC_DIRECT | Endoplasmic reticulum-Golgi intermediate compartment | 6 | 1.4E-5 | 1.6E-2 |
| GOTERM_BP_DIRECT | Retrograde vesicle-mediated transport, Golgi to ER | 6 | 3.7E-5 | 5.2E-2 |
| UP_KEYWORDS | ER-Golgi transport | 6 | 3.9E-5 | 4.8E-2 |
| GOTERM_CC_DIRECT | ER to Golgi transport vesicle membrane | 4 | 1.7E-3 | 2.0E0 |
| GOTERM_BP_DIRECT | COPII vesicle coating | 4 | 2.8E-3 | 3.9E0 |
| UP_KEYWORDS | Protein transport | 9 | 3.2E-3 | 3.8E0 |
| GOTERM_CC_DIRECT | Golgi membrane | 9 | 5.1E-3 | 5.9E0 |
| GOTERM_BP_DIRECT | ER to Golgi vesicle-mediated transport | 5 | 6.3E-3 | 8.6E0 |
| GOTERM_CC_DIRECT | Transport vesicle | 4 | 9.0E-3 | 1.0E1 |
| GOTERM_CC_DIRECT | Golgi apparatus | 10 | 1.5E-2 | 1.7E1 |
| GOTERM_CC_DIRECT | Endoplasmic reticulum-Golgi intermediate compartment membrane | 3 | 3.4E-2 | 3.4E1 |
| UP_KEYWORDS | Golgi apparatus | 8 | 4.5E-2 | 4.4E1 |
| GOTERM_BP_DIRECT | Transport | 5 | 7.6E-2 | 6.7E1 |
| GOTERM_BP_DIRECT | Intracellular protein transport | 4 | 9.4E-2 | 7.6E1 |
| UP_KEYWORDS | Cytoplasmic vesicle | 5 | 1.4E-1 | 8.3E1 |
| Cluster 9 | Enrichment score: 2.32 | |||
| UP_KEYWORDS | Lipid metabolism | 9 | 3.6E-4 | 4.4E-1 |
| UP_KEYWORDS | NADP | 5 | 6.8E-3 | 8.1E0 |
| UP_KEYWORDS | Steroid biosynthesis | 3 | 8.8E-3 | 1.0E1 |
| UP_KEYWORDS | Lipid biosynthesis | 4 | 2.5E-2 | 2.7E1 |
| Cluster 10 | Enrichment score: 2.11 | |||
| GOTERM_MF_DIRECT | Dolichyl-diphosphooligosaccharide-protein glycotransferase activity | 3 | 7.8E-4 | 9.8E-1 |
| GOTERM_CC_DIRECT | Oligosaccharyltransferase complex | 3 | 8.8E-4 | 1.0E0 |
| GOTERM_BP_DIRECT | Protein N-linked glycosylation via asparagine | 3 | 1.4E-2 | 1.9E1 |
| KEGG_PATHWAY | N-Glycan biosynthesis | 3 | 4.0E-2 | 3.6E1 |
| UP_KEYWORDS | Glycosyltransferase | 4 | 7.0E-2 | 5.9E1 |
After analyzing 84 CB2 specific interacting proteins (including CB2) with DAVID (https://david.ncifcrf.gov/home.jsp) 20 clusters were identified of which 10 were represented in this table with an enrichment score >2.