Fig. 4.
TeNT and bafilomycin (BA1) reduce the response of NR-GFP cells. A, Histograms reporting the number and relative percentage of NR-GFPcells responsive to astrocyte stimulation under different experimental conditions (see Results for details). B, Synaptobrevin immunoreactivity was reduced in Western blots of TeNT-treated astrocytes as compared with untreated astrocytes (−47.5 ± 6.3%; n = 2; Gel Doc 2000 multianalyst densitometric assay; Bio-Rad, Hercules, CA). A protein stain of the nitrocellulose revealed no differences in amount and pattern of proteins loaded. GFAP immunoreactivity was unaffected by TeNT.