Table 1.
EC50 and Hill coefficients for NMDA and glycine in wild-type and cysteine mutant NR1-1a/NR2A receptors
| NMDA | Glycine | Imax(nA) | |||
|---|---|---|---|---|---|
| EC50(μm) | n | EC50(μm) | n | ||
| NR1-1a/NR2A | 39.4 ± 1.7 | 1.82 ± 0.15 | 2.48 ± 0.12 | 1.19 ± 0.06 | 539 ± 78 |
| NR1-1a(C744A,C798A)/NR2A | 12.4 ± 0.6 | 1.17 ± 0.06 | 1.73 ± 0.13 | 1.03 ± 0.07 | 721 ± 95 |
| NR1-1a(C79S,C308S)/NR2A | 48.9 ± 2.3 | 2.00 ± 0.20 | 2.62 ± 0.09 | 1.26 ± 0.05 | 342 ± 57 |
| NR1-1a/NR2A(C87A,C320A) | 24.2 ± 1.5 | 1.55 ± 0.12 | 0.92 ± 0.04 | 1.31 ± 0.07 | 378 ± 122 |
| NR1-1a(C79S,C308S,C744A,C798A)/NR2A(C87A,C320A) | 5.95 ± 0.22 | 1.47 ± 0.08 | 0.43 ± 0.03 | 1.17 ± 0.07 | 131 ± 31 |
NMDA-evoked currents were measured in wild-type or mutant NR1-1a/NR2A receptors expressed in oocytes. EC50values ± SD were calculated from dose–response curves generated by fitting the data with the equation I =Imax/[1 + (EC50/[agonist])
F1-n
], wheren is the Hill slope, and Imax is the maximum response normalized to 100%.