Table 1.
Four-step extraction of Aβ in nontransgenic and Tg2576 transgenic mouse brain
| Tg/NTg | Age (months) | n | Aβ | TBS | 1% Triton-100 | 2% SDS | 70% FA | Total | Total Aβ (pmol/gm) |
|---|---|---|---|---|---|---|---|---|---|
| NTg | 5 | 3 | Aβ42 | ND | ND | 1.3 ± 0.1 (22) | ND | 1.3 (22) | 5.9 |
| Aβ40 | ND | ND | 4.6 ± 0.2 (78) | ND | 4.6 (78) | ||||
| Tg | 5 | 5 | Aβ42 | 0.4 ± 0.1 (1) | 3.0 ± 0.5 (7) | 6.4 ± 0.6 (15) | ND | 9.8 (23) | 42.9 |
| Aβ40 | 2.1 ± 0.2 (5) | 10.4 ± 0.9 (24) | 20.6 ± 2.5 (48) | ND | 33.1 (77) | ||||
| Tg | 6 | 5 | Aβ42 | 0.3 ± 0.1 (0.7) | 2.9 ± 0.3 (7) | 8.7 ± 1.6 (20) | ND | 11.9 (27) | 43.4 |
| Aβ40 | 1.7 ± 0.4 (4) | 9.3 ± 2.0 (21) | 20.5 ± 1.8 (47) | ND | 31.5 (73) | ||||
| Tg | 7 | 5 | Aβ42 | 0.2 ± 0.0 (0.4) | 2.3 ± 1.4 (4) | 11.6 ± 3.5 (22) | 0.9 ± 0.2 (2) | 15.0 (28) | 52.9 |
| Aβ40 | 1.3 ± 0.1 (2) | 8.0 ± 0.7 (15) | 25.5 ± 1.4 (48) | 3.1 ± 0.8 (6) | 37.9 (72) | ||||
| Tg | 21 | 3 | Aβ42 | 5.4 ± 1.3 (0.03) | 34.8 ± 6.6 (0.2) | 2042.2 ± 102.1 (10) | 482.4 ± 86.9 (2.4) | 2564.8 (13) | 19937.1 |
| Aβ40 | 7.1 ± 1.4 (0.04) | 89.6 ± 11.8 (0.4) | 11,155.6 ± 624.0 (56) | 6120.0 ± 374.0 (31) | 17,372.3 (87) |
Numbers in parentheses show the percent of total Aβ in the fraction. ND, Not detectable.