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. 2001 Jan 15;21(2):550–558. doi: 10.1523/JNEUROSCI.21-02-00550.2001

Fig. 1.

Fig. 1.

Generation of transgenic mice overexpressing Cdk5 using the p35 promoter (TgCdk5 mice). A, Diagrammatic representation of the transgene construct showing p35 promoter region (1.2 kb) ligated to the Cdk5 gene (4.8 kb). A 45 bp tag derived from SV40 containing XhoI site is inserted intoSpeI site. Sites for XbaI (X), NotI (N), EcoRI (E), and SpeI (S) are indicated. B, Genotyping of tail DNA from WT and TgCdk5 mouse by Southern blot analysis. Additional bands derived from the transgene were revealed in TgCdk5 mice. C, Northern blot analysis of Cdk5 mRNA from the entire brain showing a marked increase of Cdk5 mRNA level in the TgCdk5 mouse. D, Western blot analysis of Cdk5 protein from the entire brain showing a marked increase of Cdk5 protein in the TgCdk5 mouse. Note that the level of both the p35 and p25 proteins remain unaltered in TgCdk5 compared with the corresponding WT control.