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. 2001 Jan 15;21(2):550–558. doi: 10.1523/JNEUROSCI.21-02-00550.2001

Fig. 3.

Fig. 3.

Generation of TgKO mice. A, Genotyping of tail DNA from WT, TgCdk5, and TgKO mouse by Southern blot analysis. In the TgKO mouse, a band (arrow i) from endogenous Cdk5 was missing, and additional bands derived from the transgene (arrow ii) and mutant allele (arrow iii) were revealed. B, Northern blot analysis of Cdk5 mRNA from cerebrum, cerebellum, and spinal cord of TgKO mouse and WT mouse. Cdk5 mRNA in these brain regions from the TgKO mouse is markedly increased. C, Western blot analysis of Cdk5 protein from TgKO mouse and WT mouse. Cdk5 was overexpressed in cerebrum, cerebellum, and spinal cord in TgKO compared with the WT mouse, whereas the p35 and p25 levels are similar in both the TgKO and WT mouse. D, Cdk5 kinase activity of TgKO mouse compared with the WT mouse. In the TgKO mouse, the level of Cdk5 activity in the cerebrum, cerebellum, and spinal cord is approximately half of the corresponding WT mouse. Note that the kinase activity in the cerebrum, cerebellum, and spinal cord of WT mouse is considered as 100%.