Fig. 4.
Immunofluorescence staining in the MS for ChAT (A, F), p75 (B, G), and double-labeling of ChAT/p75 (C, H) after intracerebroventricular injections of saline (A–C) or anti-murine-p75-SAP (F–H). Immunofluorescence staining for noncholinergic neurons using calbindin (D, I) and parvalbumin (E, J) after intracerebroventricular injections of saline (D, E) or anti-murine-p75-SAP (I, J) is shown. ChAT-positive and p75-positive neurons in the MS are lost after immunotoxin injections, whereas there is no apparent loss of noncholinergic calbindin and parvalbumin staining in the septum.