Fig. 6.

Release evoked by hypertonic sucrose solution and nerve stimulation share the same vesicle pool. A, A train of nerve stimulations (20 V, 0.1 msec at 100 Hz for 200 msec) was applied, followed at 300 msec after the train by a puff application of sucrose solution (2 m in the pipette) for 500 msec. The EPSC evoked by the train was truncated to see the mEPSCs clearly. Calibration also applies to B. B, The EPSC is evoked by a puff the same as in A but without a conditioning train of stimulation. C, Shown are the EPSCs evoked by two identical electrical trains (20 V, 0.1 msec at 100 Hz for 200 msec) applied to the nerve with an interval of 1 sec (left). The EPSCs evoked by the second train (right, top) were smaller than those evoked by the first train (right, bottom). The stimulation artifacts were blanked.