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. 2001 Oct 15;21(20):7954–7968. doi: 10.1523/JNEUROSCI.21-20-07954.2001

Fig. 2.

Fig. 2.

Comparative alignment of the amino acid sequences deduced from PCR products amplified using sense primers common to both myr 8a (clone 4) and cDNA KIAA0865 and antisense primers unique to either 3′-untranslated sequence of myr 8a or KIAA0865. PCR amplifications were performed using cDNA prepared from granule neurons isolated from postnatal day 10 rat cerebellum. Sequence 1 was generated using a sense primer common to both myr 8a and KIAA0865 with an antisense primer unique to 3′-untranslated sequence of myr 8a. This product is identical to the 3′ terminus of myr 8a (clone 4) including the stop codon. Sequence 2 was generated using the sense primer common to both myr 8a and KIAA0865 with the antisense primer unique to the KIAA0865 sequence. This product does not encode a stop codon, therefore extending the sequence of myr 8a (clone 4) in the C-terminal direction. This extended sequence, which identifies the initial segment of the C-terminal tail domain of myr 8b, is 71% identical to the sequence described for the human cDNA KIAA0865. The sense and antisense primers correspond to the nucleotides of the amino acids indicated inbold text.