Fig. 6.

DOI does not change the coupling of 5-HT_1A receptors to G-proteins in the hypothalamus.A, Inhibition of [³H]8-OH-DPAT (0.8 nm) binding in hypothalamic homogenates with increasing concentration of GTPγS (from 10⁻⁹to10⁻⁵m). Each datapoint represents the mean of three assays. Specific binding is defined by WAY100635 (1 μm). B, DOI does not alter the GTPγS-induced inhibition of [³H]8-OH-DPAT binding at the GTPγS concentrations of 0.02 μm (EC₅₀) and 1 μm (E_max). The data represent the means ± SEM of [³H]8-OH-DPAT binding from eight hypothalamic samples per group; two-way ANOVA and Newman–Keuls multiple range test.