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. 2001 Oct 15;21(20):8108–8118. doi: 10.1523/JNEUROSCI.21-20-08108.2001

Fig. 3.

Fig. 3.

GDNF-c17.2 and MT-c17.2 cells engraft well and disperse within the striatum by 1 month after grafting.A–D, ISH showed the presence of cells expressing very high levels of GDNF mRNA in the GDNF grafted striatum (B, D) but not in the contralateral side (A, C). Note the abundance and dispersion of the signal in the grafted striatum (B). At higher magnification and bright field (C, D), it is possible to observe one endogenous GDNF-expressing cell (arrowhead in C) and many grafted cells with high levels of GDNF mRNA expression (D). E, G, The presence of MT-c17.2 cells was verified by β-Gal immunohistochemistry against thelacZ product (α-Lac) at 30 d after grafting. β-Gal-immunoreactive cells (magnified in G) were detected in the ipsilateral striatum to the graft (E). F, H, GDNF-c17.2 cells were also detected 1 month after grafting by GDNF immunohistochemistry.F, GDNF-c17.2 cells (magnified inH) displayed a similar distribution to cells expressing high levels of GDNF mRNA. Scale bars: (inA) A, B, E, F, 500 μm; (inC) C, D, 100 μm; (in G)G, H, 100 μm.