Glutamate/α7 nAChR double labeling at CA1 stratum radiatum synapses. A, A glutamate-like immunoreactive synaptic terminal (1,arrow, 10 nm particles) contacts a postsynaptic spine (s). The synaptic membranes show α7 nAChR-LIR (arrowhead, 5 nm particles).B, A presynaptic terminal in a glutamate-labeled preparation (2) reveals no glutamate labeling at presynaptically located vesicles (arrow). The synaptic membranes, however, show labeling for α7 nAChR (arrowhead, 5 nm particles). Scale bar (shown inB for A and B): 190 nm.