Fig. 6.

Time course of 8-OH-DPAT-induced hypothermia in NK1−/− mutants compared with NK1+/+ wild-type mice. Wild-type (A) and knock-out (B) mice received a subcutaneous injection of vehicle (▵, 10 ml of 0.9% NaCl/kg) or 8-OH-DPAT (○, 0.25 mg/kg; ●, 0.5 mg/kg), and body temperature was measured at 5 min intervals for up to 60 min. Change (in °C) in body temperature relative to baseline values (immediately before treatment, 0 on abscissa) is the mean ± SEM of determinations in six mice in each group. At both doses tested, body temperature was significantly (p < 0.05; Student's Newman–Keuls multiple t test) less in 8-OH-DPAT-treated than in vehicle-treated mice for the 10–60 min interval after injection in NK1+/+ as well as NK1−/− mice.