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. 2001 Oct 15;21(20):8026–8033. doi: 10.1523/JNEUROSCI.21-20-08026.2001

Fig. 2.

Fig. 2.

In vivo studies of the expression of ASIC mRNA levels in inflamed conditions and after action of NSAIDs.A, In situ hybridization experiments on DRG with ASIC1a and ASIC3 probes. It presents L4 DRG on the inflamed side of the animal (middle), contralateral non-inflamed DRG (left), and DRG from inflamed animal treated with aspirin (right). Arrowheads show examples of unlabeled (white) and labeled (black) cells (on 5 animals at least). B shows semiquantitative RT-PCR results. Top, Representative experiments showing RT-PCR in normal (−) and inflamed (+) conditions (−NSAID) and after treatment with NSAID (+NSAID, here aspirin). Bottom, Induction factors (mean ± SEM) of mRNA levels by inflammation (Freund) and after inflammation plus treatment with dexamethasone or NSAIDs (n = 3–10 animals per experiment). Band densities are measured and normalized to that of actin (act) in the same preparation, and the induction factor corresponds to the ratio of the normalized densities in treated over untreated conditions.