Fig. 6.
GT currents in VRAs show high Glu affinity for l-glutamate. A, Representative GT current traces induced by a series of Glu concentrations in a P10 VRA. B, Current amplitudes of the steady-state Glu-induced GT currents at different concentrations of Glu were normalized to the corresponding response evoked by 1 mm Glu in the same cell. Each data point represents the mean from three cells. Error bars show SEMs. Smooth linegives the best fit according to the Hill equation (see Materials and Methods), yielding an EC50 of 4 ± 1.1 μm and nH of 0.6.