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. 2001 Oct 15;21(20):8082–8090. doi: 10.1523/JNEUROSCI.21-20-08082.2001

Fig. 5.

Fig. 5.

Gene expression profiles, enzyme activities, and DNA ladder gel analysis in MILON and control mice of different ages.a, Results from phosphoimager quantification of Northern blots to determine mitochondrial Sod2, Gpx, Bax, Bcl-xL, Gapdh, and mtRNA transcript levels in neocortex of control (C;black bars) and MILON (M; white bars) mice at the age of 2, 4, and 5 months. Transcript levels were normalized to 18S rRNA and are presented as the percentage of the mean value of age-matched controls. **p < 0.01; ***p < 0.001. b, Northern blot analysis of transcript levels in neocortex from controls (C) and MILON (M) mice at different ages. The same blot was reprobed to detect different transcripts as indicated in the panel. c, Sod2 and Gpx enzyme activities in neocortex from controls (C; black bars) and MILON (M; white bars) mice at different ages. Values are presented as the percentage of the mean enzyme activity of age-matched controls. d, PCR DNA ladder gel assay of DNA prepared from neocortex of MILON (M) and control (C) mice at different ages. DNA fragmentation is present in neocortex of 5- to 6-month-old symptomatic MILON mice, as indicated by arrowheads. A staurosporine-treated human osteosarcoma cell line 143B was used as a positive control.