Fig. 4.

Cortical neurons with huntingtin immunoreactivity in HD mice. a, HD46 mouse (L14, 14 months, RR = 14, +clasp, hypoactive, broad-based gait) has diffuse nuclear huntingtin labeling in layer 5 pyramidal neurons. Some neurons have marked blebbing of the plasma membrane (arrows).b, Nuclear inclusions in pyramidal cells of HD100 mouse (L59, 7 months, RR = 15, +clasp). One of the neurons (top) also has diffuse nuclear labeling. c, d, Layer 6 from a transgenic control mouse with 18 glutamines in huntingtin (c, L3, 7 months, normal behavior) and an HD100 mouse (d, L59, 7 months, RR = 15, +clasp). Compared with the neurons in c, the neurons ind are reduced in size and show increased huntingtin immunoreactivity in nuclei. cc, Corpus callosum. Staining for huntingtin was performed with antibody Ab585. Scale bars:a–d, 25 μm.