Fig. 3.

Immunofluorescence of HEK cells cotransfected with GABA_A receptor subunits. HEK cells were cotransfected with α_1*, β₃, and γ₂subunits (A–F) or with α₁, β₃, and γ₂ subunits(G–I). Immunofluorescence was performed using α₁(1–9) antibodies (A, D, G), β₃(1–13) antibodies (B, E, H), or γ₂(1–33) antibodies (C, F, I) on the cell surface (A–C, G–I) or in permeabilized cells(D–F) by confocal laser microscopy (single sections). Scale bar, 10 μm. The experiment was performed four times with similar results.