Fig. 6.

Histograms of the bead-binding behavior on rat DRG growth cones loaded with either control mouse IgG or the anti-α-adaptin antibody (AP.6). An L1-Fc-coated bead was held in position against the plasma membrane of either the leading edge (defined as the region within 0.5 μm of the lamellipodial edge) or the C-domain for 3–4 sec and then released. The bead behavior was categorized as cytoskeletal association, Brownian motion, or no membrane attachment. Data from three independent experiments were pooled, and probability of the binding behavior of a total of 50–54 beads is shown. The p values between two groups were calculated from a Mann–Whitney U test, with a Kruskal–Wallis test (nonparametric ANOVA) confirming that there was a significant difference at the p = 0.0001 level.