Table 3. Comparison of Acetamide Concentration to Milk Yield and Intake of AFEX pellets.

	Murrah buffalo (Trial 1)		Karan–Fries cattle (Trial 2)
	low AFEX	high AFEX	low AFEX	high AFEX
baseline levels of acetamide in milk before treatment is initiated (ppm)a	0.34 ± 0.08	0.34 ± 0.08	0.25 ± 0.05	0.25 ± 0.05
average level of acetamide in milk (ppm)b	6.37 ± 2.41	9.59 ± 5.09	4.11 ± 1.48	5.74 ± 2.92
median level of acetamide in milk (ppm)	6.19	8.40	4.33	6.14
acetamide trend (ppm/day)c	0.081	–0.054	–0.104	–0.116
p-value for acetamide trend	0.245	<0.001	0.002	<0.001
milk yield (kg/day)	6.11 ± 1.68	6.71 ± 1.79	7.76 ± 2.43	9.93 ± 2.42
AFEX feed intake (kg/day)	2.91 ± 0.58	6.27 ± 1.36	3.22 ± 0.61	5.87 ± 1.30
acetamide in milk as a percentage of acetamide intake (%)d	0.27% ± 0.13%	0.21% ± 0.14%	0.20% ± 0.12%	0.19% ± 0.13%

^aAmount of acetamide present in milk in experimental animals prior to introduction of AFEX pellets.

^bAverage concentration of acetamide in milk for 3 (cattle) or 4 (buffalo) weeks on AFEX diet following the 14 days of initial transition to the AFEX diet.

^cLinear trend in the change of acetamide over time during the 3 or 4 weeks after the initial transition to the AFEX diet.

^dAcetamide present in the milk as a fraction of the amount present in the AFEX pellets in the feed.