Table 1. Dissociation Constants of Peptide-Mediated DNA Origami Dimers Inferred from the Relative Numbers of Complexes Observed in Electron Micrographs as Functions of Total Monomer Concentrations.
|
Kd (nM)a |
|||
|---|---|---|---|
| valency, n | complex An:Bn | complex An:An | complex Bn:Bn |
| 1 | 130 ± 20 | ||
| 2 | 25 ± 3 | ||
| 3 | 0.7 ± 0.1 | 24 ± 4 | 20 ± 3 |
a
See SI supporting discussion of experimental errors: there is an additional systematic error of approximately 20% in all Kd values corresponding to uncertainty in determining DNA origami concentrations.