Table 3.
Validation and Uncertainty Parameters of for LC-MS/MS Method.
| Compound | Linear Regression | R 2 | Recovery | LOD/LOQ (mg/kg) | U95 (%) | QPHH-IM (mg/kg) | MFH-C (mg/kg) |
|---|---|---|---|---|---|---|---|
| Salicylic acid | y = +0.2121x + 0.04 | 0.99 | 94.3 | 0.7/3.5 | 18.2 | 60.4 | 4.4 |
| Caffeic acid | y = +0.2543x + 0.01 | 0.96 | 92.8 | 1/5.0 | 20.6 | 6.0 | <LOQ |
| Kaempferol | y = +0.0095x − 0.00 | 0.96 | 93.3 | 0.3/1.5 | 12.1 | 15.2 | <LOQ |
| Penduletin | y = +0.1385x − 0.00 | 0.99 | 100.1 | 0.6/3.1 | 7.8 | 1.2 | <LOQ |
| Apigenin | y = +0.1329x + 0.05 | 0.98 | 99.7 | 1.1/6.0 | 10.8 | 5.9 | <LOQ |
| Acacetin | y = +0.6369x + 0.07 | 0.98 | 95.3 | 1.2/6.0 | 5.7 | 7.0 | 2.5 |
| Luteolin | y = +0.2217x + 0.03 | 0.98 | 99.8 | 0.7/3.5 | 4.2 | 3.7 | <LOQ |
| Diosmetin | y = +1.1820x + 0.32 | 0.98 | 100.2 | 0.6/3.0 | 3.8 | 0.7 | <LOQ |
| Taxifolin | y = +0.0735x + 0.000 | 0.97 | 91.8 | 3.1/15.0 | 10.1 | 4.2 | <LOQ |
| Eupatilin | y = +0.5231x + 0.07 | 0.98 | 96.2 | 0.9/4.0 | 15.7 | <LOQ | <LOQ |
| Nepetin | y = +0.3282x − 0.06 | 0.98 | 100.1 | 2.2/11.0 | 10.6 | 0.6 | <LOQ |
Abbreviations: LC-MS/MS, liquid chromatography–tandem mass spectrometry; LOD, limit of detection; LOQ, limit of quantification; QPHH-IM, Ida Mountains Quercus pyrenaica honeydew honey; MFH-C, Canakkale multifloral honey.