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. 2019 Sep 20;10:2097. doi: 10.3389/fmicb.2019.02097

FIGURE 4.

FIGURE 4

Effects of simvastatin on cytokine production in infected and uninfected peripheral mononuclear cells. (A) Effects of simvastatin on cytokine production in non-infected PBMCs. Cytokine production was measured in the supernatant of PBMCs incubated for 24 h with 1 μM simvastatin acid (SA1 μM), 20 μM simvastatin acid (SA20 μM), 1 μM simvastatin (S1 μM), and 20 μM simvastatin (S20 μM). The control cells were untreated cells exposed to vehicle (0.024% DMSO and 0.056% EtOH in PBS) (No Tx), or cells treated with phorbol myristate acetate (25 ng/mL) plus ionomycin (1 μg/mL). (B) Effects of simvastatin on cytokine production in infected PBMCs. The culture boxes were incubated in the previously described conditions for 24 h and subsequently, infected with M. tuberculosis H37Rv at an MOI of 0.1. At 24 h post-infection, the supernatant was collected, and cytokine production was measured, n = 10 (Kruskal–Wallis, ∗p < 0.05, ∗p < 0.01).