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. 2019 Sep 20;10:2097. doi: 10.3389/fmicb.2019.02097

FIGURE 5.

FIGURE 5

Effects of simvastatin on apoptosis in infected with Mycobacterium tuberculosis and uninfected peripheral mononuclear cells. The induction of apoptosis was evaluated in PBMCs treated for 24 h under the following conditions: 20 μM simvastatin acid (SA20 μM), untreated cells exposed to vehicle (0.024% DMSO and 0.056% EtOH in PBS) (No Tx), cells infected at an MOI of 0.1 (IFX), and cells infected at an MOI of 0.1 and treated with 20 μM simvastatin acid (IFX SA 20 μM). PBMCs treated with 100 μM etoposide for 8 h were used as a positive control for the evaluation of apoptosis. (A) Percentage of PBMCs positive for CR(DEVD)2; (B) percentage of lymphocytes positive for CR(DEVD)2; (C) percentage of monocytes positive for CR(DEVD)2; (D) percentage of NK cells positive for CR(DEVD)2; (E) percentage of NKT cells positive for CR(DEVD)2, n = 10 (Kruskal–Wallis, ∗p < 0.05, ∗p < 0.01).