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. 2019 Sep 20;10:2097. doi: 10.3389/fmicb.2019.02097

FIGURE 6.

FIGURE 6

Effects of simvastatin on autophagy in infected and uninfected peripheral mononuclear cells. The induction of autophagy was evaluated in PBMCs treated for 24 h under the following conditions: 20 μM simvastatin acid (SA20 μM), untreated cells exposed to vehicle (0.024% DMSO and 0.056% EtOH in PBS) (No Tx), cells infected at an MOI of 0.1 (IFX), and cells infected at an MOI of 0.1 and treated with 20 μM simvastatin acid (IFX SA 20 μM). PBMCs treated with 500 nM rapamycin for 8 h were used as a positive control for the evaluation of autophagy. (A) Percentage of PBMCs positive for CYTO-ID; (B) percentage of lymphocytes positive for CYTO-ID; (C) percentage of monocytes positive for CYTO-ID; (D) percentage of NK cells positive for CYTO-ID; (E) percentage of NKT cells positive for CYTO-ID, n = 10 (Kruskal–Wallis, ∗p < 0.05, ∗p < 0.01).