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. 2019 Sep 20;10:2088. doi: 10.3389/fmicb.2019.02088

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Copyright © 2019 Kong, Zhang, Wang, van der Lee, Waalwijk, van Diepeningen, Brankovics, Xu, Xu, Chen and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The FgPex3 gene replacement construct. (A) Schematic representation gene disruption strategy. FgPex3 is denoted by an open arrow; hygromycin resistance cassette (hph) is represented by a red arrow. The annealing sites of primers are indicated by small black arrows. (B) PCR analysis of PH-1 and FgPex3 deletion mutant using primers R7/R8, H850/H852, H852/R16, and R15/H850, respectively. 1–4: PCR products amplified with the above primers using PH-1 as template; 5–8: PCR products amplified with the above primers using ΔFgPex3 as template. (C,D) Southern blot hybridization analyses of transformants with hybridization probe R11/R12 (FgPex3) and R13/R14 (hph).