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. 2019 Sep 20;10:2088. doi: 10.3389/fmicb.2019.02088

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Copyright © 2019 Kong, Zhang, Wang, van der Lee, Waalwijk, van Diepeningen, Brankovics, Xu, Xu, Chen and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peroxisomal targeting fusion proteins in spores (left) and mycelia (right) of PH-1 and ΔFgPex3. The fluorescence of GFP-PTS1 and PTS2-DsRed localized to peroxisomes as discernable structures in the wild type strain PH-1, whereas both types of fluorescence exhibited a diffuse pattern in the cytoplasm of the mutant ΔFgPex3. DIC, differential interference contrast; GFP-PTS1, green fluorescent protein contained in the peroxisomal targeting signal 1; DsRed-PTS2, red fluorescent protein contained in the peroxisomal targeting signal 2; Merge, overlays of green and red fluorescence proteins images. Scale bar = 10 μm.