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. 2019 Sep 20;10:2088. doi: 10.3389/fmicb.2019.02088

FIGURE 4.

FIGURE 4

Growth analysis of F. graminearum strains under different culture conditions. (A) The wild-type PH-1, deletion mutant and the complemented strain were cultured on three basic media (PDA, CM, and MM) as well as MM plates supplemented with osmotic stress agents (NaCl, KCl, LiCl, sorbitol or glucose), MM plates amended with either cell wall damaging agents (SDS, Congo red, caffeine or calcofluor white) or with various carbon sources (2% sucrose, 40 mM sodium acetate (C2), 40 mM sodium butyrate (C4), 40 mM sodium valerate (C5), 40 mM sodium hexanoate (C6), 3 mM laurate acid (C12), 3 mM tridecanoic acid (C13), 3 mM myristic acid (C14), 3 mM palmitic acid (C16) or 3 mM oleic acid (C18) as the sole carbon source) at 25°C for 3 days. (B–D) Colony diameters of the tested strains were measured to calculate the inhibition rate and statistically analyzed. Error bars represent the deviation from five replicates, and the same letter on the bars for each treatment indicates no significant difference at P = 0.05.