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. 2019 Sep 20;10:2088. doi: 10.3389/fmicb.2019.02088

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Copyright © 2019 Kong, Zhang, Wang, van der Lee, Waalwijk, van Diepeningen, Brankovics, Xu, Xu, Chen and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduced ability of ΔFgPex3 to scavenge reactive oxygen species (ROS) survival assay. (A) Tetranitroblue tetrazolium chloride (NBT) staining for ROS accumulation in cultures of ΔFgPex3 and the wild-type. In plates at the bottom 0.6 M NaCl was added to promote ROS production. The dark blue color of the colony indicated ROS accumulation. (B) Strains PH-1,ΔFgPex3, FgPex3C were inoculated onto CM plates and incubated for 7–90 days. Mycelial plugs from the cultures were transferred to fresh CM agar plates, and the viability of the culture was observed. DAI, days after incubation.