Skip to main content
. 2019 Aug 12;13(9):9980–9992. doi: 10.1021/acsnano.8b09137

Figure 6.

Figure 6

Tag charge dependence of the threshold voltage and translocation probability. (a) Every additional positive charge in the fusion tag of the DHFRNtagO2 variants increases the threshold voltage (see Supporting Information S26) by ≈5.21 mV. The solid line is a linear fit to the data. (b) Translocation probability voltage VPtranslbias plotted against tag charge for Ptransl = 0.1, 5, 50, 95 and 99.9% shows that variants with high tag charge require less bias voltage to fully translocate the pore. Values were obtained through interpolation from eq 8, using the parameters in Table 2.