Table 1. Mutations and Charges of All DHFR Variants.
| mutationsa |
protein charge at pH 7.5 [e] |
||||
|---|---|---|---|---|---|
| name | body (res. 1–163) | tag (res. 164–190) | body | tag | total |
| DHFR4S | –10 | +4 | –6 | ||
| DHFR4I | V88E P89E | –12 | +4 | –8 | |
| DHFR4C | A82E A83E | –12 | +4 | –8 | |
| DHFR4O1 | E71Q | –12 | +4 | –8 | |
| DHFR4O2 | T68E R71E | –13 | +4 | –9 | |
| DHFR5O1 | E71Q | A175K | –12 | +5 | –7 |
| DHFR7O1 | E71Q | A175K A174K A176K | –12 | +7 | –5 |
| DHFR5O2 | T68E R71E | A175K | –13 | +5 | –8 |
| DHFR6O2 | T68E R71E | A175K A174K | –13 | +6 | –7 |
| DHFR7O2 | T68E R71E | A175K A174K A176K | –13 | +7 | –6 |
| DHFR8O2 | T68E R71E | A175K A174K A176K A169K | –13 | +8 | –5 |
| DHFR9O2 | T68E R71E | A175K A174K A176K A169K L177K | –13 | +9 | –4 |
a
With respect to DHFR4S.