Fig. 7.

Optimization of the riboflavin operon by improved CRISPR/Cas9n mediated multiplex gene editing. a Integration of the riboflavin synthesis pathway genes and regulatory elements. b Production of riboflavin in 96-well plates. The red bar shows the control strain. c Yield of riboflavin in 96-well plates. Striped bar show strains that were selected for secondary screening due to high yields, which were not among the 20 highest-producing strains. The data show the average values and standard deviations of triplicate experiments