Figure 2.
Testing PhoX peptide enrichment in a complex background. (a) Sensitivity—mixing cross-linked BSA peptides in decreasing amounts in a fixed background of E. coli peptides provides an estimate of the detection limit of the methodology. (b) Recovery method—mixing cross-linked BSA peptides in equal amounts in an increasing background of E. coli peptides allows for recovery of cross-linked BSA peptides upon enrichment. The estimated cross-link amounts for the given cross-linked BSA peptides are shown above the bars.