Figure 4.

SD leads to decreased AnkG staining. A, Confocal images obtained from coronal cortical slices maintained under control conditions (top) or subjected to two consecutive hypoxic episodes without (middle) or with (bottom) synaptic blockers in the bath. The sections were stained for the AIS cytoskeletal protein, AnkG (cyan), the neuronal marker, NeuN (red), and DAPI (white). The almost complete absence of AIS AnkG immunoreactivity in neurons that have recovered from SDs (Post SD). By contrast, AnkG immunosignal is largely preserved in slices that were treated with synaptic blockers to prevent SD. B, Number of AnkG-positive AISs counted in 1 mm² section in control (gray) and posthypoxia (yellow) slices without and with (blue) synaptic blockers. C, Ratio of the number of AnkG-stained AISs to the number of NeuN-stained neuronal somata in control slices, recovered slices, and slices exposed to synaptic blockers. Box charts represent median (horizontal line), 25th-75th percentile (box), and standard deviation (bars). n = 7, n = 10, and n = 8 slices, respectively. *p < 0.05, **p < 0.01. ns indicates p > 0.05.