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RLC converts anti-inflammatory monocytes to a proinflammatory subset in vitro. A, A gating strategy to identify monocyte subsets in the CD11b+ splenocyte culture. B, Percentage of Ly-6C subsets and the ratios of Ly-6Chigh over Ly-6Clow subset. Sera from sham or RLC mice were collected and treated to the naive splenocytes. t test: **p < 0.01; ***p < 0.001.
