Figure 3.

Spatial and temporal distribution of zebrafish pnpo. The expression profiles of pnpo in zebrafish at different developmental stages and tissues in adult fish were examined by RT-PCR and WISH. Embryos at the (A) indicated stages and (B) tissues of adult female zebrafish were extracted for total mRNA and subjected to RT-PCR with zpnpo-specific primers. The amount of zpnpo transcripts was normalized with zebrafish β-actin. Results presented are the averages shown in mean ± SEM from at least three independent repeats. (C) Embryos at various developmental stages were examined with WISH by probing with the DIG-labeled riboprobes specific to zpnpo as described in the Materials and Methods. RT-PCR, reverse transcription–polymerase chain reaction; WISH, whole-mount in situ hybridization; DIG, digoxigenin.