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. 2019 Sep 20;10:1086. doi: 10.3389/fphar.2019.01086

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Copyright © 2019 Chen, Tu, Schirch, Safo and Fu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The locomotor activity of zPnpo morphants at 4 dpf. Zebrafish larvae with the indicated treatment were placed individually in each well on a 48-well plate and rested in the tested chamber for 5 min before recording. (A) The swimming track, representing larval cumulative location in 1 min, was recorded. (B–E) Larval swimming behavior was recorded for 30 min and analyzed for velocity (B), maximum acceleration (C), body activity (D), and absolute turning angle (E) with the built-in analytic software in DanioVision High-throughput tracking system. Reported are the averages of data from at least three independent repeats with n = 30∼164 embryos for each group and expressed as mean ± SEM. The statistical significance was calculated with one-way ANOVA (Kruskal–Wallis test) by comparing the experimental groups with wild type (*) or with zPnpo MO (#). ^# p < 0.05; ^## p < 0.01; ***/^### p < 0.001. MO, morpholino oligonucleotide; ANOVA, analysis of variance.