FIGURE 9.

Production of oxygen and nitrogen reactive species by A549 infected-cells with isolates of A. baumannii. The oxygen reactive species (superoxide anion) was quantified at 3 h post-infection (A) and the nitrogen reactive species (nitric oxide) at 24 h post-infection (B). Each open circle corresponds to the average of two independent experiments, each one in duplicated (n = 4). Each bar is plotted as the mean ± SD. Bars in upper panels indicate the superoxide anion (A) and nitric oxide (B) production by A549 cells infected with each isolate from the HCG, HRGIZ, and HGM-P. Heat map chart in lower panels indicates the level of superoxide anion and nitric oxide production by A549 infected cells. A. baumannii-17961 was used as a control strain of these experiments. NI, non-infected. NS, non-significant; ^∗p < 0.05.