TABLE 1.
Primers used for PCR amplification of β-lactamase and ISAba1 genes.
| Gene | Primer sequence | Amplicon length (bp) | Tannealing (°C) | References |
| Class A β-lactamase | ||||
| blaTEM | F: ATGAGTATTCAACATTTTCG R: TTACCAATGCTTAATCAGTGAG |
861 | 55 | Celenza et al., 2006 |
| blaCTX–M–type | F: CGCTTTGCGATGTGCAG R: ACCGCGATATCGTTGGT |
550 | 52 | Bonnet et al., 2000 |
| blaSHV | F: ATGCGTTATATTCGCCTGTGTATT R: TTAGCGTTGCCAGTGCTCGATC |
861 | 60 | This study |
| blaKPC | F: TCACTGTATCGCCGTCTAGTTCTG R: TTACTGCCCGTTGACGCCCAATC |
875 | 58 | This study |
| Class B β-lactamase | ||||
| blaVIM | F: GAGTGGTGAGTATCCGACAGTCAACGAAAT R: AGAGTCCTTCTAGAGAATGCGTGGGAATCT |
389 | 58 | This study |
| blaNDM | F: GTCTGGCAGCACACTTCCTATCTC R: GTAGTGCTCAGTGTCGGCATCACC |
516 | 58 | This study |
| blaIMP | F: GCATTGCTACCGCAGCAGAGTCTTTG R: GCTCTAATGTAAGTTTCAAGAGTGATGC |
647 | 58 | Alcántar-Curiel et al., 2019 |
| Class D β-lactamase | ||||
| blaOXA–40–like | F: TCTAGTTTCTCTCAGTGCATGTTCATC R: CATTACGAATAGAACCAGACATTCC |
749 | 58 | This study |
| blaOXA–51–like | F: ATGAACATTMAARCRCTCTTACTTA R: CTATAAAATACCTAATTMTTCTAA |
825 | 50 | Alcántar-Curiel et al., 2014 |
| ISAba1 | F: CACGAATGCAGAAGTTG R: CGACGAATACTATGACAC |
548 | 55 | Rosales-Reyes et al., 2017 |
| ISAba1F/OXA-51-likeR | F: CACGAATGCAGAAGTTG R: CTATAAAATACCTAATTMTTCTAA |
1380 | 50 | Turton et al., 2006; Rosales-Reyes et al., 2017 |