TABLE 1.
Bacterial strains and plasmids used in this study.
| Description | Source or reference | |
| Strains | ||
| R. anatipestifer RA-YM | R. anatipestifer wild-type strain (serotype 1) | Lab collection |
| R. anatipestifer ATCC 11845 | Standard strain of R. anatipestifer | Purchased at Guangzhou Microbial Preservation Center |
| E. coli X7213 | Thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc:Mu λpir ΔasdA4 Δzhf-2:Tn10 | Lab collection |
| Plasmids | ||
| pIC333 | Source of SpecR cassette | Lab collection |
| pRE112 | Suicide vector, sacB mobRP4 R6K ori CmR | Life Technology |
| pRE112- RAint | Suicide vector with 10K GI integrase and attachment site | This study |
| pRE112-Spec-RAint | pRE112- RAint vector with SpecR | This study |
| pRE112-Spec-eGFP-RAint | pRE112-Spec-RAint with Spec promoter and eGFP CDS | This study |