Figure 2 –

Quantification (A) and example images (B) of the immunofluorescence staining of CD8⁺ cells from the in-field and out-of-field hemi-irradiated tumors at 24 hours after irradiation with 10Gy RT (blue: DAPI, green: CD8). An average of 3 separate experiments is shown, with 21 mice per treatment group. (C) Quantification of CD8⁺ cells from: unirradiated controls, 100% irradiated and from the in-field and out-of-field halves of the hemi-irradiated tumors. A representative experiment is shown, with 5 mice per group. Experiment was performed 3 times, with a total of 15 mice per group. (D) The effects of CD8⁺ T cells depletion on 67NT tumor response to RT. CD8⁺ T cells were depleted by four IP injections of anti-CD8 antibody, at a dose of 200μg/injection, starting 3 days before RT and 0, +3, +6 days after RT. Experiment was done once, with 5 mice per treatment group.