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. Author manuscript; available in PMC: 2019 Sep 27.
Published in final edited form as: Mol Pharm. 2018 May 16;15(6):2069–2083. doi: 10.1021/acs.molpharmaceut.8b00319

Table 1:

In vivo collagenase effect on diffusion

Particle Amount of enzyme Duration of treatment Route of Injection Source Tumor Type Effect Change in ECM Ref
IgG 2000 µg 1 hour Intratumoral Bacterial collagenase (Sigma) Mu89 melonoma (dorsal skinfold chamber) 1.2X increase in percentage of particles undergoing fast diffusion N/A 46
IgG 30 mg 24 hour Intratumoral Clostridium (biochemical corp) HSTS sarcoma, U87 (dorsal skinfold chamber) 2X increase in diffusion coefficient N/A 47
150 kDa Dextran 100 µg 24 or 48 hour Tail vein Clostridiopeptidase A (Sigma) OHS osteosarcoma (dorsal skinfold chamber) 2X increase in diffusion coefficient N/A 48
500 kDa Dextran, Albumin 2000 µg 3 hour Intratumoral Bacterial collagenase (Sigma) B16F10 melanoma (shoulder blades) 2X increase in tumor diffusion coefficient (.5 mm depth).
10X increase in tumor diffusion coefficient (2 mm depth)		 Reduction by western blot 49