Table 5:
Particle | Amount of enzyme | Duration of treatment | Source | Tumor Type | Size | Effect | Ref |
---|---|---|---|---|---|---|---|
2MDa, 150 kDa Dextran | 1–10 mg/mL | 1 hour | Clostridiopeptidase A (Sigma) | OHS osteosarcoma | 150–250 uM | 70% increase in diffusion coefficient 2Mda Dextran. No change for 150 Kda particle (even after 18 hours) | 48 |
Polystyrene beads | 0.004–1 mg/mL | 5 hour | Clostridium Type I (Sigma) | SiHa cervial carcinoma | 400–500 uM | 12X increase in fluorescence intensity in spheroid core for 40 nm particle; 7X for 20 nm, 3X for 100nm particle; 1.5X for 200 nm; 4X for 100 nm collagenase coated nanoparticles |
80 |
Collagenase coated albumin nanoparticles | N/A | 96 hour | Clostridium Type I (Sigma) | C8161+ melanoma cocultured and HFF (human foreskin derived fibroblasts) | 300–400 uM | 5% increase in imaged total area of labeled particles in spheroids by 100 nm; 27% for 200 nm | 81 |
AAVP Bacteriophage vector | 100 µg | 1 hour | Clostridium Type I (Sigma) | 9L Glioma | N/A | 2.6X increase in luciferase expression, 40% decrease in cell viability (67% with combination) | 82 |