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. Author manuscript; available in PMC: 2019 Sep 27.
Published in final edited form as: Mol Pharm. 2018 May 16;15(6):2069–2083. doi: 10.1021/acs.molpharmaceut.8b00319

Table 5:

In vitro collagenase effect in tumor spheroids

Particle Amount of enzyme Duration of treatment Source Tumor Type Size Effect Ref
2MDa, 150 kDa Dextran 1–10 mg/mL 1 hour Clostridiopeptidase A (Sigma) OHS osteosarcoma 150–250 uM 70% increase in diffusion coefficient 2Mda Dextran. No change for 150 Kda particle (even after 18 hours) 48
Polystyrene beads 0.004–1 mg/mL 5 hour Clostridium Type I (Sigma) SiHa cervial carcinoma 400–500 uM 12X increase in fluorescence intensity in spheroid core for 40 nm particle; 7X for 20 nm, 3X for 100nm particle; 1.5X for 200 nm;
4X for 100 nm collagenase coated nanoparticles		 80
Collagenase coated albumin nanoparticles N/A 96 hour Clostridium Type I (Sigma) C8161+ melanoma cocultured and HFF (human foreskin derived fibroblasts) 300–400 uM 5% increase in imaged total area of labeled particles in spheroids by 100 nm; 27% for 200 nm 81
AAVP Bacteriophage vector 100 µg 1 hour Clostridium Type I (Sigma) 9L Glioma N/A 2.6X increase in luciferase expression, 40% decrease in cell viability (67% with combination) 82