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Normalized DPH fluorescence anisotropy of DSPC vesicles with increasing amounts of additives. A) Cholesterol, B) DSPE-PEG, C) DiCHEMS-PEG, and D) DiCHOL-PEG. DSPC liposomes containing sterol-anchored PEG exhibit similar anisotropy traces to liposomes with cholesterol. The mole percent of unmodified cholesterol is twice that of the mole percent of DiCHEMS-PEG or DiCHOL-PEG to have an equivalent amount of cholesterol in the bilayer. Errors bars represent mean ± SD.
